detinfДЕТСКИЕ ИНФЕКЦИИCHILDREN INFECTIONS2072-81072618-8139Association of Pediatricians and Infection Disease doctors10.22627/2072-8107-2017-16-4-17-21detinf-320Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИССЛЕДОВАНИЕ ЗНАЧИМОСТИ ОБНАРУЖЕНИЯ НУКЛЕОКАПСИДНОГО АНТИГЕНА ВИРУСА ГЕПАТИТА С У ДЕТЕЙ С ХРОНИЧЕСКИМ ГЕПАТИТОМ СINVESTIGATION OF THE SIGNIFICANCE OF DETECTION OF THE NUCLEOCAPSID ANTIGEN OF THE HEPATITIS C VIRUS IN CHILDREN WITH CHRONIC HEPATITIS CЛейбманЕ. А.LeybmanE. A.

к.м.н., младший научный сотрудник

лаборатория генно-инженерных препаратов

123098, Москва, ул. Гамалеи, 18 +7(499)190-30-01

PhD, junior researcher of laboratory of gene engineering products 

+7(499) 190-30-01

dr.leybman@gmail.comНиколаеваЛ. И.NikolaevaL. I.

дбн, ведущий научный сотрудник

лаборатория генно-инженерных препаратов

123098, Москва, ул. Гамалеи, 18

MD, leading researcher of laboratory of gene engineering products

L.i.nikolaeva@mail.ruСапроновГ. В.SapronovG. V.

кмн, старший научный сотрудник

лаборатория генно-инженерных препаратов,

123098, Москва, ул. Гамалеи, 18

доцент

кафедра инфекционных болезней

PhD, senior researcher of laboratory of gene engineering products

g_sapronov@mail.ruСамохваловЕ. И.SamokhvalovE. I.

ведущий научный сотрудник

лаборатория экологии вирусов

123098, Москва, ул. Гамалеи, 18

PhD, leading researcher of laboratory of viral ecology

e_samokh@hotmail.comКовалевО. Б.KovalevO. B.

дмн, профессор

кафедра инфекционных болезней у детей

педиатрический факультет

+7(499) 256-60-26

MD, professor of the department of infectious diseases in children

doctor87@list.ruКоневВ. А.KonevV. A.

кмн, доцент

кафедра инфекционных болезней у детей

педиатрический факультет

+7(499) 256-60-26

PhD, assistant professor of the department of infectious diseases in children

konev60@mail.ruУчайкинВ. Ф.UchaikinV. F.

дмн, академик РАН, профессор

кафедра инфекционных болезней у детей

педиатрический факультет

+7(499) 236-25-51 

academician of RAS, MD, professor of the department of infectious diseases in children

uchaikin@list.ruФГБУ «Национальный исследовательский центр эпидемиологии и микробиологии им. Н.Ф. Гамалеи» Минздрава России, МоскваРоссияNational Research Centre of Epidemiology and Microbiology named after N.F. Gamaleya, MoscowRussian FederationФГБУ «Национальный исследовательский центр эпидемиологии и микробиологии им. Н.Ф. Гамалеи» Минздрава России, Москва; ФГБОУ «Российская медицинская академия непрерывного профессионального образования», МоскваРоссияNational Research Centre of Epidemiology and Microbiology named after N.F. Gamaleya, Moscow; Russian Medical Academy of Continuing Professional Education, MoscowRussian FederationФГБОУ ВО «Российский национальный исследовательский медицинский университет им. Н.И. Пирогова» Минздрава России, МоскваРоссияRussian National Research Medical University named after N.I. Pirogov, MoscowRussian Federation2017071220171641721Copyright © Лейбман Е.А., Николаева Л.И., Сапронов Г.В., Самохвалов Е.И., Ковалев О.Б., Конев В.А., Учайкин В.Ф., 20172017Лейбман Е.А., Николаева Л.И., Сапронов Г.В., Самохвалов Е.И., Ковалев О.Б., Конев В.А., Учайкин В.Ф.Leybman E.A., Nikolaeva L.I., Sapronov G.V., Samokhvalov E.I., Kovalev O.B., Konev V.A., Uchaikin V.F.This work is licensed under a Creative Commons Attribution 4.0 License.https://detinf.elpub.ru/jour/article/view/320

Цель исследования — изучить клинико-диагностическое значение обнаружения нуклеокапсидного (core) антигена вируса гепатита С у детей с хроническим гепатитом С. Анализировали сыворотки крови 101 больных и 24 неинфицированных участников. Сore-антиген определяли хемилюминисцентным методом (Architect HCV Ag, «Abbott», США), РНК ВГС — методом ОТ-ПЦР, фиброз печени — неинвазивными методами, специфические антитела — методом ИФА. Установлена зависимость между содержанием core-антигена и РНК ВГС, субтипами вируса, уровнем АЛТ и фиброзом печени. Показаны высокая чувствительность и специфичность хемилюминисцентного метода определения core-антигена у детей с ХГС. Впервые предложены три диапазона концентраций core-антигена.

The aim of this study was to investigate the clinical and diagnostic significance of the detection of core-antigen of hepatitis C virus (HCV) in children with chronic hepatitis C (CHC). Sera of 101 patients and 24 non-infected participants were analyzed. Core-antigen was determined by chemiluminescent method using «ARCHITECT HCV Ag» (Abbott, USA). HCV RNA was detected by highly sensitive OT-PCA. The presence of liver fibrosis was established by non-invasive methods. Antibody to core-antigen was detected by ELISA using commercial test-systems. A correlation was found between concentration of core-antigen and HCV RNA, viral subtypes, ALT level, and presence of liver fibrosis. High sensitivity and specificity of the chemiluminescent method for the detection of core antigen in children with CHC was shown. For the first time three ranges of core-antigen concentrations were proposed. 

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The authors declare that there are no conflicts of interest present.