FEATURES OF INTESTINAL MICROBIOTA IN CHILDREN WITH A SYNDROME OF EXCESSIVE BACTERIAL GROWTH IN THE SMALL INTESTINE
https://doi.org/10.22627/2072-8107-2018-17-1-22-27
Abstract
The purpose of the study was to determine the features of the parietal microbiota of the intestine in children with a verified syndrome of excessive bacterial growth in the small intestine. Clinical and laboratory examination of 25 children at risk of intrauterine infection at the age of 8 months — 4 years with a verified syndrome of excess bacterial growth in the small intestine was performed based on the results of the hydrogen breath test. Investigation of the species and quantitative composition of the parietal intestinal microbiota was carried out with the help of the gas chromatography-mass spectrometry method with determination of the concentration of microbial markers by drop of blood (laboratory of bifidobacteria of the Federal Budgetary Institute of Science Moscow Research Institute of Epidemiology and Microbiology name after G.N. Gabrichevsky). It was revealed that all of them recorded a high concentration of microbial markers of gram-negative anaerobic bacteria of the colon and viruses of the Herpes family due to a deficit of representatives of priority genera (Propionibacterium Freunderherii 5-fold, Eubacterium spp. 4.8-fold, Bifidobacterium spp. 4-fold, Lactobacillus spp. 1.5-fold) with an excess of endotoxin (by 1.5—2-fold) and a decrease in plasmalogen (by 2-fold). These data testify to the inflammatory process of the small intestinal mucosa, which aggravates the disturbances in its functioning and confirm the informative nature of the gas chromatography and spectrometry method.
About the Authors
L. A. Lityaeva
Orenburg State Medical University of the Ministry of Health of Russia.
Russian Federation
Russian Federation
Lyudmila A. Lityaeva, MD, Professor of the Department of Epidemiology and Infectious Diseases.
Orenburg.
O. V. Kovalyova
Orenburg State Medical University of the Ministry of Health of Russia.
Russian Federation
Russian Federation
Oksana Kovalyova, PhD, Associate Professor of the Department of Epidemiology and Infectious Diseases.
Orenburg.
O. G. Zhilenkova
Moscow Research Institute of Epidemiology and Microbiology name after G.N. Gabrichevsky.
Russian Federation
Russian Federation
Olga Zhilenkova, PhD, Head of the Laboratory of Bifidobacteria.
Moscow.
References
1. Ursova N.I. Dysbacteriosis of the intestine in childhood: innovations in diagnosis, correction and prevention: A guide for doctors. M., 2013: 328. (In Russ.)
2. Shenderov B.A. Medical microbial ecology and functional nutrition. Т. 1: The microflora of humans and animals and its functions. М.: GRANT, 1998: 288. (In Russ.)
3. Beloborodova N.V., Osipov G.A. Homeostasis of small molecules of microbial origin and its role in the relationship between microorganisms and the host. Vestnik RAMS. 1999;16(7):25—31. (In Russ.)
4. Belousova E.A. Syndrome of excessive bacterial growth in the small intestine in the light of the general concept of intestinal dysbiosis: a look at the problem. Pharmateka. 2009;2:10—11. (In Russ.)
5. Method of mass spectrometry of microbial markers as a method for assessing the parietal intestinal microbiota in diseases of the digestive system: Teaching-methodical manual. Under the editorship of G.A. Osipov, V.P. Novikova. SPb., 2013: 96. (In Russ.)
6. Mubarakshina O.A. Excessive bacterial growth in the intestine: features of pathogenesis and pharmacotherapy. Med.Vestnik. 2008; 19: 446. (In Russ.)
7. Osipov G.A., Fedosova N.F., Lyadov K.V. Qualitative in situ microbiological analysis of lipid markers in biological fluids using gas chromatography-mass spectrometry. Zdravoohranenie i Medicinskie Tekhnologii=Healthcare and Medical Technology. 2007; 5: 20— 23. (In Russ.)
8. Osipov G.A., Demina A.M. Chromatomasspectrometric detection of microorganisms in anaerobic infectious processes. Bulletin of the Russian Academy of Medical Sciences. 1996; 13 (2): 52—59. (In Russ.)
9. Osipov G.A., Parfenov A.I., Verkhovtseva N.V., Ruchkina I.N. et al. Clinical study of microorganisms of the intestinal mucosa by culturebiochemical and chromatography-mass-spectrometric methods. Ext. Wedge. Gastroenterology. 2003, 4: 59—67. (In Russ.)
10. Osipov G.A. Chromato-mass-spectrometric analysis of microorganisms and their communities in clinical samples in infections and dysbiosis. Chemical analysis in medical diagnostics. M.: Science, 2010: 293—368. (In Russ.)
11. Assessment of the microecological status of a person using chromatography-mass spectrometry. New medical technology. Registered in Roszdravnadzor for №НЮ-40006 of August 17, 2009. (In Russ.)
12. Kinross J.M. von Roon, A.C. Holmes, E, Darzi, A. Nicholson J.K. The human gut microbiom implication for future health care. Current Gastroenterology Reports. 2008;10:396—403.
13. Ouwehand A. The role of intestinal microflora for the development of the immune system in early childhood. Eur J.Nutr. 2002; suppl.I:32—37.
14. Paul J. Small intestinal Bacterial Overgrowth: Histopathologic Features and Clinical Correlates in an Underrecognized Entity. Archives of Pathology and Laboratory Medicine: February, 2010; 134(2):264—270.
15. Plotnikova E.YU., Zaharova YU.V. Diagnosis and treatment of the syndrome of excessive bacterial growth. Russian Medical Journal=Russkij Mediciskij Zhurnal. 2015;13:767—770. (In Russ.)
Review
For citations:
Lityaeva L.A., Kovalyova O.V., Zhilenkova O.G. FEATURES OF INTESTINAL MICROBIOTA IN CHILDREN WITH A SYNDROME OF EXCESSIVE BACTERIAL GROWTH IN THE SMALL INTESTINE. CHILDREN INFECTIONS. 2018;17(1):22-27. (In Russ.) https://doi.org/10.22627/2072-8107-2018-17-1-22-27
Views:
1188
Email this article 