Etiological role and molecular-genetic features of Streptococcus pneumoniae in children’s infectious diseases
I. N. Protasova,
G. P. Martynova,
N. A. Ilyenkova,
I. A. Kutischeva,
S. V. Domracheva,
O. P. Ovchinnikova,
E. S. Sokolovskaya https://doi.org/10.22627/2072-8107-2020-19-1-7-12
Abstract
Objective: To study the etiological role, serotype distribution and prevalent Streptococcus pneumoniae genotypes (sequence types) in 459 children hospitalized with acute purulent otitis media, acute tonsillitis, community-acquired pneumonia, and purulent bacterial meningitis. Pneumococcal cultures isolated from patients were tested for sensitivity to antimicrobials, and presence of resistance genes.
Materials and Methods: The cultural and molecular methods (PCR, sequencing) were used. Statistical analysis was carried out using Microsoft Excel 2007 and Statistica 6.0.
Results: S. pneumoniae was detected in 16,8%. The prevalent serotypes were 19F (39.7%) and 19A (13.2%) — representatives of multidrug-resistant clonal com-plex 320. Isolates of serogroup 6 (6AB, 8.82%) belonged to clonal complex 315, and characterized by resistance to macrolides, clindamycin and tetracycline.
From 70.6% to 88.2% of S. pneumoniae serotypes isolated from patients corresponded to «vaccine»-types.
About the Authors
I. N. Protasova
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Irina N. Protasova - PhD, Associate Professor of Microbiology Department, Professor V. F. Voino-Yasenetsky Krasnoyarsk State Medical University.
Krasnoyarsk.
G. P. Martynova
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Galina P. Martynova - MD, Professor, Professor V. F. Voino-Yasenetsky Krasnoyarsk State Medical University.
Krasnoyarsk.
N. A. Ilyenkova
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Natalya A. Ilyenkova - MD, Professor, Professor V. F. Voino-Yasenet-sky Krasnoyarsk State Medical University.
Krasnoyarsk.
I. A. Kutischeva
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Irina A. Kutishcheva - PhD, Associate Professor of Children’s Infec-tious Disease Department, Professor V. F. Voino-Yasenetsky Krasnoyarsk State Medical University.
Krasnoyarsk.
S. V. Domracheva
Krasnoyarsk Interregional Children’s Hospital № 4
Russian Federation
Russian Federation
Svetlana V. Domracheva - Clinical Pharmacologist, Krasnoyarsk Interregional Children’s Hospital №4.
Krasnoyarsk.
O. P. Ovchinnikova
Krasnoyarsk Interregional Children’s Clinical Hospital №1
Russian Federation
Russian Federation
Olga P. Ovchinnikova - Head of Bacteriological Laboratory, Clinical Pharmacologist, Krasnoyarsk Interregional Children’s Hospital №4.
Krasnoyarsk.
E. S. Sokolovskaya
Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University
Russian Federation
Russian Federation
Elena S. Sokolovskaya - Reseacher of Children’s Disease De-partment, Professor V. F. Voino-Yasenetsky Krasnoyarsk State Medical University.
Krasnoyarsk.
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Review
For citations:
Protasova I.N., Martynova G.P., Ilyenkova N.A., Kutischeva I.A., Domracheva S.V., Ovchinnikova O.P., Sokolovskaya E.S. Etiological role and molecular-genetic features of Streptococcus pneumoniae in children’s infectious diseases. CHILDREN INFECTIONS. 2020;19(1):7-12. (In Russ.) https://doi.org/10.22627/2072-8107-2020-19-1-7-12
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