Changes in the Structure of the Chromosomes of Lymphocytes during Persistent Infection in Children
https://doi.org/10.22627/2072-8107-2016-15-4-30-32
Abstract
Children with chronic diseases of respiratory tract were investigated for IgM and IgG serum antibodies to viruses (Herpes simplex virus, Epstein-Barr virus, Cytomegalovirus) and bacteria (Chlamydia trachomatis + pneumoniae, Mycoplasma pneumonia) by ELISA and for telomere length of their lymphocytes by fluorescent hybridization in situ followed by flow cytomerty (FlowFISH). The purpose of the study was to compare the lymphocyte telomere length of these seropositive and seronegative children. Regression analysis and analysis of percent distribution relative to the regression line have compensated age differences between seropositive and seronegative groops and demonstrated shorter telomeres in lymphocytes of children those have serum antibodies to viruses in all combinations (including combinations with antibodies to bacteria) in contrast to seronegative children of the same age.
About the Authors
V. S. Sevryukova
The Joint Stock Company «DiLUCH» Health Resort Complex
Russian Federation
Anapa
Russian Federation
Anapa
E. O. Khalturina
First Moskow State Medical University after I.M. Sechenov
Russian Federation
Moscow
Russian Federation
Moscow
P. E. Dobryakov
The Joint Stock Company «DiLUCH» Health Resort Complex
Russian Federation
Anapa
Russian Federation
Anapa
V. S. Kozhevnikov
The Joint Stock Company «DiLUCH» Health Resort Complex
Russian Federation
Russian Federation
professor, consultant at laboratory of clinical immunology,
353440, Krasnodarskyi Krai, g-k Anapa, 22 Pushkina str.
References
1. Blackburn E.H. Structure and function of telomeres // Nature. 1991; 350: 569—572.
2. Aubert G., Lansdorp P.M. Telomeres and Aging // Physiol. Rev. 2008; 88: 557—579.
3. Rufer N., Dragowska W., Thornbury G., Roosnek E., Lansdorp P.M. Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry // Nat. Biotechnol. 1998; 16: 743—747.
4. Zeichner S.L, Palumbo P., Feng Y.R., Xiao X. et all. Rapid Telomere Shortening in Children // Blood. 1999; 93: 2824—2830.
5. Effros R.B. Telomere/telomerase dynamics within the human immune system: Effect of chronic infection and stress // Experimental Gerontology. 2011; 46: 135—140.
6. Epel E.S., Blackburn E.H., Lin J., Dhabha F.S., Adler N.E., Morrow J.D., Cawthon R.M. Accelerated telomere shortening in response to life stress // Proc. Nat. Acad. Sci. USA. 2004; 101: 4917312—4917315.
7. Côté H.C.F., Soudeyns H., Thorne A. et al. Leukocyte Telomere Length in HIV-Infected and HIV-Exposed Uninfected Children: Shorter Telomeres with Uncontrolled HIV Viremia // PLOS ONE. 2012; July 16 (online).
8. Plunkett F.J., Soares M.V.D., Annels N., Hislop A., Ivory K, Lowdell M., Salmon M., Rickinson A, Akbar A.N. The flow cytometric analysis of telomere length in antigen-specific CD8 T cells during acute Epstein-Barr virus infection // Blood. 2001; 97: 700—707.
9. Борисов В.И., Кожевников В.С., Сенюков В.В., Сизиков А.Э. и др. Укорочение длины теломер моноцитов при ревматоидном артрите // Мед. иммунология. 2006; 1: 87—90. Borissov V.I., Kozhevnikov V.S., Senyukov V.V., Sizikov A.E. et all. [Shortening Telomere Length of Monocytes in Rheumatoid Arthritis] // Med. Immunology. 2006; 1: 87—90. (In Russ).
10. Deng Z., Wang Z. and Paul M. Lieberman P.M. Telomeres and viruses: common themes of genome maintenance // Front. Oncol. 2012; 31 December (оnline).
11. Van de Berg P.J., Griffiths S.J., Yong S.-L., Macaulay R. et al. Cytomegalovirus Infection Reduces Telomere // J. Immunol. 2010; 184: 3417—3423.
Review
For citations:
Sevryukova V.S., Khalturina E.O., Dobryakov P.E., Kozhevnikov V.S. Changes in the Structure of the Chromosomes of Lymphocytes during Persistent Infection in Children. CHILDREN INFECTIONS. 2016;15(4):30-32. (In Russ.) https://doi.org/10.22627/2072-8107-2016-15-4-30-32
Views:
925
Email this article 